Epithalon (Epitalon)
Molecular Research Profile ยท Target purity > 99.5% ยท Half-life Short
Telomerase-activating tetrapeptide researched for biological age reversal, pineal gland regulation, and circadian rhythm normalization.
Epithalon (Epitalon) Molecular Formula, Structure & Chemical Identity
Reference identifiers researchers use to verify that a vial matches what a vendor claims to have shipped. Cross-check these values against any Certificate of Analysis before accepting a purity figure.
| Molecular Formula | C14H22N4O9 |
|---|---|
| Molecular Weight | 390.4 g/mol |
| CAS Number | 307297-39-8 |
| Amino Acid Count | 4 |
| Amino Acid Sequence | Ala-Glu-Asp-Gly |
| Sequence (1-letter) | AEDG |
| Also Known As | Epitalon, Epithalone, AEDG peptide |
| PubChem CID | 219042 ↗ |
Identifiers verified against the NIH PubChem compound database. Molecular weights independently recalculated from the molecular formula using IUPAC 2021 standard atomic weights.
How to Read a Epithalon (Epitalon) Certificate of Analysis
A COA is only meaningful if the identifiers on it match the compound you ordered. Four checks, in order.
- 1. Identity โ does the formula match?The COA header should state C14H22N4O9 and a molecular weight near 390.4 g/mol, with CAS 307297-39-8. A mismatch means you are not looking at a COA for this compound.
- 2. Purity โ HPLC chromatogram, not just a numberA printed “> 99.5%” with no chromatogram is a marketing claim. Demand the actual HPLC trace: a single dominant peak, labelled retention time, and an integration table. Shoulders or unlabelled side peaks are unreported impurities.
- 3. Mass confirmation โ does MS agree with the formula?Mass spectrometry should report an observed mass consistent with the expected 390.4 g/mol. Peptides are frequently reported as multiply charged ions, so the raw m/z value will be lower than the neutral mass. An unexplained offset is a red flag.
- 4. Provenance โ who signed it, and for which batch?A valid COA names the testing laboratory, carries a batch or lot number that matches your vial label, and is dated. A vendor-produced PDF with no lab identity and no batch number is not third-party evidence.
Full methodology in ourguide to the 5 COA red flags researchers miss, or verify the reconstitution math with thepeptide reconstitution calculator.
Mechanism of Action
Epithalon (Ala-Glu-Asp-Gly) is a synthetic tetrapeptide designed to mimic the active region of epithalamine, a polypeptide extract from the pineal gland. Its primary proposed mechanism is activation of telomerase โ the enzyme responsible for extending telomeres, the protective caps at chromosome ends that shorten with each cell division and serve as a molecular clock of cellular senesence.
In addition to telomerase activation, epithalon has been shown to regulate melatonin and circadian rhythm gene expression, modulate pineal peptide secretion, influence the hypothalamic-pituititary axis, and reduce oxidative stress markers in aged animal models. Research from the St. Petersburg Institute of Bioregulation and Gerontology has reported lifespan extension and reduced age-related pathology in rodent and primate studies.
The peptide's effects appear to be cycle-dependent rather than continuous โ a 10โ20 day administration cycle repeated every 4โ6 months is the typical research protocol, rather than ongoing daily administration.
Research History & Context
Epithalon was developed in the 1990s by Prof. Vladamir Khavinson at the St. Petersburg Institute of Bioregulation and Gerontology, as part of a broader program of peptide bioregulators derived from tissues of young animals. It has been studied in over 50 preclinical and clinical publications, primarily from Russian research institutions.
A 15-year observational study in elderly human subjects (published 2003โ2018) reported reduced all-cause mortality and lower rates of age-associated disease in the epithalon-treated cohort compared to controls. However, independent replication outside of the originating research group remains limited, and rigorous double-blind trials have not been completed by Western research institutions as of 2026.
Storage & Handling
Store lyophilized epithalon at -20ยฐC in a dark, dry environment. Stability data is more limited than for other research peptides; conservative protocols recommend use within 12 months of lyophilization even when frozen.
After reconstitution with bacteriostatic water, epithalon should be refrigerated at 2โ8ยฐC and used within 14 days โ a shorter window than many other peptides due to limited stability data. Some researchers reconstitute with sterile water for injection (rather than BAC water) for immediate use only, discarding any remaining solution. Freeze-thaw of reconstituted epithalon is not recommended.
Reconstitution & Research Dosing
For Epithalon (Epitalon), the standard laboratory reconstitution is 2.0 ml BAC water for 10mg vial. A typical research study window uses 5 - 10 mg / daily (10-20 day cycle).
For a 10mg epithalon research vial, add 2.0 ml BAC water (5.0 mg/ml). Due to the short recommended in-solution stability window (14 days), plan research cycles so that each reconstituted vial is consumed within this period. For a 10-day protocol at 10 mg/day, reconstitute one 10mg vial per day using sterile water for injection and use immediately.
Use the Reconstitution Calculator for exact syringe units →Audited Vendors Stocking Epithalon
Each review checks whether the testing lab is named and whether COAs can be verified at the issuing lab.
Related Epithalon Research
Epithalon FAQ
QWhat is the molecular formula of Epithalon (Epitalon)?
Epithalon (Epitalon) has the molecular formula C14H22N4O9 and a molecular weight of approximately 390.4 g/mol. Its CAS registry number is 307297-39-8. It is a 4-residue peptide with the sequence Ala-Glu-Asp-Gly.
QHow do you verify a Epithalon (Epitalon) Certificate of Analysis?
Check four things in order. First, identity: the COA must state C14H22N4O9 and CAS 307297-39-8. Second, purity: require the actual HPLC chromatogram with retention time and integration table, not just a printed percentage. Third, mass confirmation: the mass spectrometry result must agree with the expected molecular weight of 390.4 g/mol, allowing for multiply charged ions. Fourth, provenance: the COA must name the testing laboratory and carry a batch number matching your vial label.
QWhat purity should Epithalon (Epitalon) be?
Research-grade Epithalon (Epitalon) should meet a purity target of > 99.5%, confirmed by third-party HPLC analysis rather than a vendor self-declaration. A purity claim without an accompanying chromatogram carries no evidential weight.
QHow much bacteriostatic water do you add to Epithalon (Epitalon)?
The standard reconstitution for Epithalon (Epitalon) is 2.0 ml BAC water for 10mg vial. For a 10mg epithalon research vial, add 2.0 ml BAC water (5.0 mg/ml). Due to the short recommended in-solution stability window (14 days), plan research cycles so that each reconstituted vial is consumed within this period. For a 10-day protocol at 10 mg/day, reconstitute one 10mg vial per day using sterile water for injection and use immediately.
Explore Related Research Compounds
Research Use Only (RUO): Epithalon (Epitalon) is discussed strictly as an in-vitro laboratory research compound. Nothing on this page constitutes human or clinical administration guidance. Always verify purity through independent third-party HPLC/MS analysis before use.